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Publicações
Projetos em que participa

José Carlos Roseiro

Categoria

Investigador Principal com Agregação

Academic degrees and titles

2004 - Title “Agregado” (Habilitation, Fermentation Technology) by the University of Évora, Portugal.

1991- Doctor of Philosophy, University of Birmingham, UK.

1985 - Master of Science in Process Biotechnology, University of Birmingham, UK.

1981 - First degree (licenciatura). Pharmacy, Faculty of Pharmacy, University of Lisbon. Portugal

Activities / Research Interests

Current appointments

Head of the Quality Coordination Group of LNEG Accredited Laboratories. Appointed by the LNEG Directive Board by the Decision 5/2009.

Head of the Ethics Committee of LNEG. Appointed by the LNEG Directive Board by the Decision 5/2011.

Head of the Laboratory Waste Management Committee. Appointed by the LNEG Directive Board at 11 June 2011.

Research interests

Microbial responses to environmental changes in steady state cultures

The stress introduced in the steady state population, by the lack of nutrient creates an under population ignored by the traditional means of representing microbial growth. The cells, that are statistically put aside of the nourished steady state population, depolarise by the lack of chemical energy and stop growing until it gets a nutrient supply and recover. This fact conveys the cell back to the active population. For a given nutrient concentration an equilibrium constant defining a rate of cell change between the active and dormant population is observed. Thus, a constant presence of a dormant population deviates the culture from a reliable steady state

Scale up effects on microbial physiology

Insufficient mixing in industrial level bioreactors leads to broth gradients affecting microbial metabolism and the overall culture efficiency. Oxygen transfer deficiency leads to shifts in metabolism whereas nutrient concentration gradients results in culture limitation and yield drop. In industrial media, concentration gradients produces the local accumulation of toxic compounds, namely short chain organic acids leading to acid toxicity, activating the proton translocating membrane ATPase with the consequent decrease of available energy for growth and biosynthesis.

Applications to Bioenergy

Microbiological production of lactic acid and fuels in hemicellulose hydrolysate feeds. A two-step process can be run as separate hydrolysis and fermentation (SHF) or as simultaneous saccharification and fermentation (SSF) are used. Lactic acid is used to produce biodegradable and biocompatible polylactate polymers, being an environment-friendly alternative to conventional non-biodegradable plastics derived from petrochemicals. These approach aims at reducing the amount of petrol diverted towards the chemical industry. Second generation Ethanol is produced by fermenting sugars obtained from enzymatic hydrolysis of agro-industrial substrates along with high value products.

Selected publications

Patents

Process for producing Xanthan using non-metabolizable organic acids. Número de patente: WO9505476  (Patente Mundial), Data de publicação: 1995-02-23, Inventores: Roseiro, J; Collaço, M. Propriedade: Instituto Nacional de Engenharia e Tecnologia Industrial NERA; AIDA.

An yeast microbial consortium, its production process and its utilization in white wine production. Referência: Candidatura PT, Propriedade: Instituto Nacional de Engenharia e Tecnologia Industrial, Universidade de Évora, Collaço, M; Gírio, F; Albergaria, M.; Xufre, A; Roseiro, J; Laureano, P; Rosário, F; Amorim, L; Martins, M.

Selected publications in peer review journals

Feio, S, Nunes, L, Pereira, P, Silva, AM, Roseiro, JC, Gigante, B, Marcelo-Curto, MJ (2007) Antifungal activity of dehydroabietic acid derivatives on wood contaminant fungi. Journal of Microbiological Methods, 70, 465-470.

Caldeira AT, Feio S, Santos-Arteiro JM, Feio S, Coelho A, Roseiro JC (2008) Environmental dynamics of Bacillus amyloliquefaciens CCMI 1051 anti-fungal activity under different nitrogen patterns, Journal of Applied Microbiology, 104, 808–816.

Teresa Lopes da Silva, Lucie Piekova, João Mileu, José Carlos Roseiro (2009) A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures, Enzymeand Microbial Technology, 45,134-138.

 Teresa Lopes da Silva, Daniela Feijão, José Carlos Roseiro, Alberto Reis(2011) Monitoring Rhodotorula glutinisCCMI 145 physiological response and oil production growing on xylose and glucose using multi-parameter flow cytometry, Bioresource Technology, 102, 2998-3006.

Teresa Lopes da Silva, José Carlos Roseiro and Alberto Reis (2012). Applications of Multi-parameter Flow Cytometry to Microbial Biofuels Production Processes. Trends in Biotechnology, 30, 225-232. doi:10.1016/j.tibtech.2011.11.005.